The Technology

MicroSort® is a sperm separation method based on the difference in the amount of genetic material in sperm. Sperm carrying an X chromosome has approximately 2.8% more DNA material than sperm carrying a Y chromosome.

During the MicroSort® process, the sperm sample is washed to remove seminal liquid and nonmotile cells. After the washing, the sample is stained with a special fluorescent material that attaches to the DNA contained in the sperm.

The stained sperm cells are analyzed one by one by a flow cytometer, in which cells pass through a laser to make the stain attach to the DNA fluoresce. The sperm containing the X chromosome (which have more DNA and therefore more stain) will shine brighter than the sperm containing the Y chromosome.

The flow cytometer uses a special software to identify X and Y chromosome sperm based on their fluorescence signature. The sperm carrying the chromosome that will produce the desired gender are separated from the rest of the sample — resulting in an enriched sperm sample ready for use.

The sorting process of each semen sample takes between 6 and 7 hours. Many assisted reproductive techniques allow MicroSort® to cryopreserve the samples for future use or for shipment to any part of the world.

Contact our exclusive Microsort centers

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call: (703) 621-7171
Business Hours: 10:00 – 15:00 Central Time (GMT -5:00)